In cases of divergences, the evaluation was repeated using double-headed microscope.> Statistical analysis of the results took advantage of Statistica 98 PL software (Statsoft, Poland).
Although designed for non-divergence free velocity fields, we show that the basic IIOE scheme is locally mass conservative in case of divergence free velocity.
The values of ϵ are limited by a lower bound of 10−5, and in case of divergence of the iterative solver for (epsilonle10^{-5}) a direct solver is used instead.
In case of divergence, a third researcher will be consulted and a consensus will be obtained through discussion.
The process and provisional analysis will be triangulated among investigators and, in case of divergence; consensus will be obtained through discussion.
In individual cases of divergence, consensus had to be achieved.
Mismatch as a result of single nucleotide polymorphisms can decrease the signals of single probes and influence the mean probe set signal leading to a higher standard deviation as shown here and in cases of strong sequence divergence to systematically lower signal intensities [ 23, 30].
COI delivered a species identification for 98.8% of species, using either a distance-based criterion or, in cases of very low divergence (less than 0.5%), using diagnostic nucleotide substitutions.
In cases of extreme sequence divergence, Phyre [ 39] was used to evaluate potential secondary structure.
