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In case of extraction of total glutenin subunits, the pellet was eventually extracted with a solution (1 mg: 10 μl) of 50% propanol containing 50 mM Tris-HCl pH 8.8, 1 mM EDTA, 10 mM iodoacetamide or 1.4% of 4-vynilpyridine, 1% (w/v) DTT for 1 h at 70°C.
The antioxidant in case of extraction methods and among plant parts found significantly different (P < 0.05). in case of shaking extraction method, leaves showed higher TPC and TFC values followed by seed, fruit, stem and roots, whereas in case of DPPH the trend was as; stem > leaves > seeds > roots > fruit.
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In cases of extraction, retraction using a CLR with temporary skeletal anchorage devices (TSADs) has the advantages of esthetically favorable results and early soft tissue change [2, 3].
In addition, the formation of densified zones (increased solids holdup) close to the membrane wall that was observed in case of gas extraction may increase the bed-to-membrane mass transfer resistance.
In case of shaking, extraction was performed for 6 h at room temperature (Shaker Gallenkamp, UK).
In case of RNA extraction for microarray experiment, 400 μl of Trizol® reagent (Invitrogen) was added to the frozen plant tissue and the sample was ground to homogeneity before an additional 600 μl of Trizol® was added.
After a final pelleting step at 100,000×g for 1 hour, purified exosomes were resuspended in 50 100 μL of PBS and processed immediately (in case of RNA extractions) or stored at 4°C for a maximum of 5 days (for macrophage uptake experiment and Nanosight analysis).
This has already been shown in the case of extraction in flow through a packed coffee bed [5].
The analysis in the case of extraction from a packed coffee bed is the subject of a separate paper [20].
The physical description of extraction here is observed to describe extraction well in the case of extraction from a dilute suspension of coffee grains.
In the case of extraction of coffee from a suspension of coffee grains, unlike the system for extraction from a packed coffee bed, we have conservation of coffee in the system.
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