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Here we show that the three genes, CO, FT and SOC1 are up-regulated in brm mutant lines (Figure 1) raising the question of whether BRM controls these genes dependently or independently of each other.
However, not only is expression of MP-regulated target genes reduced in brm syd mutants, but it is also increased (FIL) upon BRM or SYD complex tethering (via MPN-BSH).
Interestingly, levels of H3K27me3 at the FLC promoter were reduced in brm plants with respect to WT plants (Figure 3C), suggesting that BRM can cooperate directly or indirectly with the Polycomb complex in repressing FLC in non-vernalized conditions.
Because the bandwidth specified in BRm- 1,x is allocated, the BS is only required to schedule the newly generated traffic.
Most of the groups used modeling, as it is included in BRM and it is new for them, which was because they used to think experiment as the only way for a scientific activity.
Therefore, the difference of the bandwidths specified in BRm- 1,x and BR m,n is the traffic generated during the sleep period after BRm- 1,x was issued.
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The enhanced early flowering was even more extreme in short days (23.7±2.8 and 34.2±10.1 leaves in brm-1 and flc-3 plants, respectively, versus 17.4±1.45 leaves in brm-1 flc-3).
In BRM-silenced plants, CO expression is still limited to the veins, although a clear up-regulation is observed.
These results were confirmed in brm-1 and brm-2 mutant plants by RT-PCR experiments (Figure 1A).
Furthermore, the levels of FLC mRNA were significantly increased in brm-1 both in LD and SD (Figure 3B).
FT is up-regulated in brm-1 and flc-3 mutants, but this up-regulation is stronger in the flc-3 brm-1 plants and the same was observed for SOC1 (Figure 2B 2C).
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