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In both the constructs, molecular mass of the plant-expressed CtxB was 14.6 kDa in contrast to 11.6 kDa for the authentic CtxB.
The fluorescence emitting from GFP and CFP proteins in both the constructs co-localized.
In both the constructs, the N-terminal end of the α-element was pasted to the C-terminal end of the N-element.
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In preliminary experiments both the constructs were used to express BCO in E. coli BL21 DE3 pLysS cells grown in LB medium, by inducing the protein expression with 1 mM IPTG at different times of growth and collecting the cells (grown at 25 or 37°C) 3, 7 or 18 hours after induction.
In both the previous examples, we constructed a COSTBC for the N = 2-hop case by repeatedly using the same OSTBC at both the source and the relay stage.
This stability increases confidence both in the construct measured by the GABS and in the ability to assess levels of gambling affinity across disparate ranges of gambling-problem severity.
Based on the latest embodied energy intensity database for the Chinese economy 2007, the embodied energy of a typical constructed wetland in both the construction and operation stages is accounted and compared with those for a cyclic activated sludge plant as a typical conventional wastewater treatment system and a hybrid system of conventional wastewater treatment system and constructed wetland.
A dimerization-disrupting mutation (A206K, [19]; here denoted as nd for non-dimerizing) was therefore introduced in both FPs in the construct (CFPnd-linker-YFPnd; Figure 2).
The upstream GFP and the F2A (20aa) were identical in both constructs: the only difference was the short "linker" between GFP and F2A created by the cloning strategy: -RAK RSLE- (pGFP-F2A20-CherryFP; linker derived from furin and XhoI restriction site) and -SGS RGAC- (pGFP2AGUS; linker derived from XbaI and SphI restriction sites), with only the arginine (shown in bold type face) in common.
After 16 weeks of in vivo implantation, both the engineered cartilage constructs and the bare scaffolds were found to be filled the defects with white cartilaginous tissue, with the engineered constructs showing histologically good subchondral bone connection and better surrounding cartilage infusion.
After treatment with CpG methylase, the relative luciferase activities were significantly decreased in the constructs both with and without the DR-1 and DR-2 elements (** P < 0.005, Fig. 5b).
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