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Antibodies produced in these cell lines are enriched in bisected and non-fucosylated oligosaccharides and are more potent than their counterparts produced in the wild-type CHO cell lines in mediating ADCC towards target cells [69].
The triple knockdown of Gsc and Vent1/2 led to the surprising result of the restoration of normal embryonic patterning, not only in whole embryos but also in bisected dorsal and ventral half-embryos.
We recently observed the dynamic movements of posterior chamber-associated structures, e.g., the lens capsule, zonular fibers, and anterior hyaloid membrane during cataract surgery using view technique in bisected porcine eyes [ 24].
We have previously found that the glycosyltransferase, GnT-III (encoded by the MGAT3 gene) (Nishikawa et al, 1992), which is the sole biosynthetic enzyme for bisecting GlcNAc modification (Bhattacharyya et al, 2002), is upregulated in the brains of AD patients (Akasaka-Manya et al, 2010), but how this increase in bisected glycan contributes to AD pathology remained unclear.
Three glycoengineering approaches have been used (i) over expression of the enzyme GntIII in the antibody producing cells, which results in the Fc segment containing an increase in bisected non-fucosylated oligosaccharides, (ii) producing antibody in CHO cells that lack the transferase enzyme involved in core-fucosylation and (iii) using siRNA to knockdown fucosyl transferase activity.
Taken together, these results implicate Pitx2 as a good candidate for an upstream regulator of cVg1 expression: it is a transcription factor, it is expressed in the same domain as cVg1 in whole embryos and in bisected embryo marginal zone, and it is expressed before cVg1.
Similar(52)
All sentinel nodes were fixed in formalin, bisected, embedded in paraffin, and cut at a minimum of six levels at 50- to 150-μm intervals.
At 0, 248 48 and 72 h, tumors were fixed in 10% formalin in PBS, bisected, then each half was embedded in paraffin and sectioned.
For Akt detection, wandering L3 larvae were rinsed in PBS, bisected, and the anterior half was everted and placed in 100 μl oxygenated Buffer A on ice (Ceddia et al., 2003).
Both cars carve out ample space for two adults in back, bisected by consoles that disallow a third person.
The eight-member teams on Sunday fielded four raiders and four stoppers inside a big circle 140 feet in diameter bisected midway by a white line.
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