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Interestingly, a glutamic acid (depicted in blue) present in Bcd is conserved in all the MEIS subfamily members.
Upon this signal, a bistable mechanism allows for large changes in hb promoter occupancy under small changes in Bcd concentration across some threshold generating an on off expression pattern.
Another difference lies at the level of the 4EHP-interacting sequence which is 66YNYIRPYL73 in Bcd and 59YRHPLFPLL67 in Prep1 (Fig. 1A), i.e. with an additional important leucine in the case of Prep1.
While both the nuclear trapping model and our current model support an important role of nuclei in Bcd gradient formation [24], [44], they differ from each other in the following fundamental aspects.
Firstly, as seen below, the residuals following signal extraction in Bcd are nonstationary.
UCd and serum ferritin explained almost 60% of the variation in BCd.
We again found that no new combinations arose in bcd RNAi (supplementary material Fig. S9).
CN2 expression was significantly higher in CC than in BCD (p < 0.006) or CM tissues (p < 0.0001).
Over the entire follow-up period, the annual decrease in BCd averaged 2.7% in the LEA (n = 258) and 1.8% in the HEA (n = 203).
The feed-forward NN model with two hidden layers has difficulties in filtering the fluctuations to accurately capture the signal in Bcd.
For the 10 wild-type cell fates lost in bcd RNAi, most cells were located in the anterior (supplementary material Fig. S7).
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