Because serum starvation may represent proapoptotic conditions for the cells as depicted in Figure 4, we next evaluated whether tumour cells could undergo caspase-dependent cell death in base medium (BSA condition) but be protected by chemokine treatments.
To establish whether CXCL9, CXCL10, CXCL11 can modulate the growth/survival ability of the colon cancer cell line in culture, we examined and quantified cell growth after plating C26 and HT29 cells for 5 days at low density in base medium alone (serum starvation) or supplemented with various concentrations of CXCL9, CXCL10 or CXCL11.
For cell glycolysis measurement, T cells seeded in XF24 microplates as described above were cultured in XF base medium supplemented with 2 mmol/L L-glutamine in CO2-free incubator for 1 h.
MCF-10A cells, derived from normal human breast epithelium and immortalized but nontumorigenic (Soule et al. 1990), were grown in a base medium (MEGM Bullet Kit; Cambrex, East Rutherford, NJ), with all additives supplied in the kit except cholera toxin.
Strains were grown in a base medium composed of 100 g/L malt extract (ME) (Sigma-Aldrich, St . Louis MO, USA).
Strains were grown in a base medium composed of 100 g/L ME (Sigma-Aldrich, St . Louis MO, USA).
The strain was grown in a base medium composed of 100 g/L ME (Sigma-Aldrich, St . Louis MO, USA) supplemented with YPD.
It is cultured in the base medium: 1 1 mix of MCDB105 and 199 with the final concentration of sodium bicarbonate 2.2 g/l.
They were cultured in the base medium: 1 1 mix of MCDB105 and 199 supplemented 15% of human serum, 0.25 U/ml of insulin, 2 mM of l-glutamine.
The particle size, shape/morphology and nanoparticle distribution in the base medium were determined by transmission electron microscopy (TEM) using a JEOL JEM2010 (JEOL Ltd., Akishima, Tokyo, Japan) operating at 200 kV with a beam current of 105 μA.
The particle size, shape/morphology and distribution of nanoparticles in the base medium were determined by transmission electron microscopy (TEM) with a JEOL JEM2010 equipment (JEOL Ltd., Akishima, Tokyo, Japan) at 200 kV and 106 μA.
