These results demonstrate the utility of recently developed receptor reconstitution techniques for assessing the functionality of purified receptors and show a direct link between a covalent modification of a membrane-bound receptor and its impaired functionality in a reconstituted system.
In order to assess the functionality of Sso1p and Sso2p phosphorylation sites the putative phosphoamino acids S23, S24 and S79 in Sso1p and S31 and S34 in Sso2p were mutagenized to alanine or glutamic acid to mimic either constitutively non-phosphorylated or phosphorylated forms of these amino acids.
Therefore, in order to assess the functionality of the cDNA library, we studied the expression of two well-characterized maize proteins, calreticulin and ABP1.
Furthermore, in order to assess the functionality of these constructs, all the truncation mutants as well as full-length hMsd1 were created in an siRNA-resistant manner (Fig 3A and supplementary Fig S3A).
Next section, we discuss how these limitations in conventional microfluidic models are improved in human organs-on-chips models to assess the functionality of NPs.
It should be noted here that young persons were selected that were acquainted with the use of PCs and mobile devices, in order to be able to assess the functionality and usefulness of the developed application, without being influenced by the possible difficulty in the use of such devices.
Following the in silico identification of putative epitopes here, work is currently underway to assess the functionality of these sites in vitro.
The aim of the present work was to assess the functionality of DiCre in vivo and its ability to be used to establish conditional deleter mice.
Further studies are underway to validate this finding in a larger, longitudinal cohort of patients and to assess the functionality of these cells.
Three basic measurements were employed to assess the functionality of the identified NSSs in all 13 mtDNA protein-coding genes and 6 nDNA-encoded mitochondrial protein-coding genes.
