An early and fundamental step for retina development is the generation of distinct retinal cell types in appropriate numbers.
Cells from exponentially growing culture were seeded in appropriate numbers in 60 × 15 mm Petri dishes with 10 ml of appropriate culture medium.
Single cell suspensions were counted and seeded in appropriate numbers in 60 × 15 mm Petri dishes (Falcon) with 6 ml appropriate culture media.
Using Chinese painted quails for vaccine studies with HPAI H5N1 viruses that require enhanced biosafety level 3 (BSL-3+) conditions provides a reasonable opportunity for the inclusion of the birds in appropriate numbers and groups in the experiments, thus achieving scientifically acceptable results.
An IP3R in which a single residue (D2550A) was mutated to create a pore that is blocked whenever its luminal surface is bathed in Ca2+ provides a channel that is effectively "pore-dead" throughout its normal life cycle as it passes from ER to PM, yet these channels were effectively counted in appropriate numbers (∼2 into/cell) inthethe PM.
The cells were placed in the appropriate number of 10 cm tissue culture plates (Corning), incubated in a 5% CO2 incubator at 37°C, expanded once and frozen in 50% FBS, 40% media, and 10% DMSO (Sigma) for long time storage and future use.
In contrast, OCSO can scales in much faster, whereas each optimization would reduce a group of VMs in an appropriate number according to the real-time utilizations.
So we learn that on arrival each soul is assessed by the monster Minos, who indicates which circle he or she must go to by arranging his tail in the appropriate number of coils.
As if piecing together a Byzantine mosaic, Olympiodorus hands his students the shards and tesserae that could amount, in the appropriate number and arrangement, to classical paideia.
