Sentence examples for in appropriate blocking from inspiring English sources

Primary antibodies were applied in appropriate blocking solution overnight at 4°C.

Secondary antibodies were applied in appropriate blocking solution: Horseradish peroxidase-conjugated-conjugated anti-rabbit/anti-mouse (Dako, Glostrup, Denmark) at 1 2000 and 1 3000, respectively, for 1 h at room temperature.

After deparaffinization and antigen retrieval in sodium citrate buffer (pH 6.0), endogenous perioxidase was quenched with 3% hydrogen peroxide in PBS for 10 min. Non-specific binding was minimized by incubation in appropriate blocking serum for 30 min and endogenous biotin was blocked with an avidin/biotin blocking kit (Vector).

Tissues were then incubated with the primary antibody (Table 1) diluted in the appropriate blocking serum, and incubated overnight in a humidified chamber at 4°C.

Primary antibodies were diluted in the appropriate blocking serum (Table 2) and incubated on sections overnight at 4°C.

Blots were blocked in PBS-T (pH 7.4, 0.1 % Tween) with 3 % BSA (Biotest) or 3%% skimmed milk (Sigma), and subsequently incubated with the indicated antibodies in the appropriate blocking buffer.

A 1 500 dilution in the appropriate blocking serum of biotinylated rabbit anti-mouse IgG (DAKO, High Wycombe, UK) for SMA and Ki-67, biotinylated rabbit anti-goat IgG (DAKO) for AMH, and biotinylated swine anti-rabbit IgG (DAKO) for AR and P450 side chain cleavage enzyme (P450scc) was used.

Part of 14 Gut designates which hole George is supposed to run in and the appropriate blocking scheme.

In order to achieve appropriate blocking volume, it is of great importance to identify and calculate steam breakthrough channel.

Frozen sections were incubated free floating with gentle agitation overnight at 4 °C in PBST and 3% of the appropriate blocking serum (Vector Laboratories) and primary antibody.

Selecting an appropriate blocking key is necessary.