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Despite being a palindromic structure, it was not involved in any predicted secondary structure.
The output from Exonerate for all four genomes was again interrogated for the presence of a start codon in any predicted gene structure.
Each of the four CARP genes identified by the RNAi screen was PCR amplified from the CpdA-resistant R0.8 cell line, cloned, and sequenced for mutations in the ORF, as well as in any predicted UTR regions.
It should be noted that the combination of the two PfamB domains together with the 'potential thioredoxin fold' in a single protein could not be identified in any predicted non-SEO proteins from the analyzed plants and appears unique to the SEO family.
The first alternative is based on the facts that the Dtnbp1 sdy allele is, strictly speaking, not a null mutation but a partial deletion that comprises two internal exons and does not create frameshift in any predicted open reading frame, and that transcripts expressed from the mutant allele have been detected at levels comparable to those expressed from the wild-type allele (Li et al., 2003).
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The C-terminal regions of the proteins contain few, if any, predicted CICPs in the region containing a high concentration of disordered residues (Fig. 2C).
However, in previous games, any predicted boost to the economy through tourism simply has not materialised.
In addition, we detected by microarray a transcriptional activity in a region without any predicted ORF.
Interestingly, the predicted FBX pseudogenes were selectively enriched in the group without any predicted recruitment modules (Fischer's exact test, p<0.05), suggesting that many of the corresponding proteins lost their ability to bind targets even if expressed (Table S6).
That could include any predicted changes in income or working hours, but might also include any plans to have children in the near future.
As such, it is likely that any predicted gradient in socioeconomic deprivation using SIMD will be an overestimation of the true inequality in life expectancies.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com