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Comparison of CD127lo and CD127hi cells from post-infusion PBMC samples in a mixed lymphocyte reaction with autologous responder and irradiated allogeneic stimulator cells demonstrate that CD127lo cells suppress thymidine incorporation in a proliferation assay by 36% in one patient and 18% in another (sorted to responder ratio of 0.3∶1, tested in duplicate) [20].
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In another experiment, sorted cells of the CA1 cell line (CD44high and CD44low subpopulations) were plated at similar density, exposed to apoptosis-inducing drugs for 48 hours, and then trypsinized and plated again at clonal density.
Hence, the process can be continued in the same fashion, outputting the hyperpaths in H in sorted order.
After choosing v i, another loop iterates over all vertices v j in the sorted neighbour list.
Without telling anyone, Kiefer began collecting the items, stashing them in sorted piles in the garages of friends.
Conversely, a list of genes detected in whole tissue but not in sorted cells was also compiled.
Cell cycle phase distribution was determined by standard Propidium Iodide staining in control bone marrow cells and in sorted, RFP-negative cells (no Mybl2 RNAi) and in sorted, RFP-positive cells (Mybl2 knockdown).
The expression of both genes was higher in sorted CD138low cells and in cells after genotoxic stress than in the main population.
However, we can not detect any Egr-2 message in sorted CD69+ thymocytes in our mice, suggesting that the deletion is very efficient.
On average, more IL-8 was detected in sorted cells than in whole tumor or tissue.
The requests in the sorted queues are processed in batches (fifo_batch).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com