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Figure 1 Network components in an Internet building block representing a hop. Figure 2 Network components in an light path building block representing a hop.
In a 25 µl PCR reaction, 2 µl of cDNA (corresponding to 20 ng of total RNA input) was amplified in an Light Cycler 480 (Roche) using 2-fold QuantiTect Multiplex PCR Master Mix (Qiagen), 50 nM primers and 100 nM probe for the 18S rRNA reference gene (fwd: 5'-CGGCTACCACATCCAAGG-3', rev: 5'-CGGGTCGGGAGTGGGT, probe: 5'-TTGCGCGCCTGCTGCCT), and 300 nM primers and 100 nM probe for the gene of interest.
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Salt bushes fanned in a light breeze.
The Singapore noodles, in a light curry sauce, were dry.
"You want it to be coated in a light film.
The scene is enveloped in a light morning mist.
I watch them drive off in a light drizzle.
Two swelling bars in, a light rain began to fall.
It's like water in a light green straw.
"Who can say?" she answers, "in a light tone".
They walked south for twenty blocks in a light rain.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com