Monomeric Aβ42 fractions were stored at 4°C and were used within three days of purification in all experiments to avoid the presence of pre-formed aggregation in our reactions.
Strains with identical auxotrophies and other genetic markers were used in all experiments to avoid phenotypic differences due to marker effects.
Only one female, randomly chosen, from each of the 200 families formed was used in this experiment to avoid pseudo-replication via a brood effect.
The impact of this would be greatest when cells are seeded at a low density, as they were in this experiment to avoid them reaching confluence before day 7.
However, a low current density (15 A/m2) was used in the experiment to avoid the hydrogen bubbles and black deposits.
Each experiment incorporated exemplars from at least four adult females (Blumstein and Daniel 2004, N = 4; Blumstein et al. 2008a, N = 8, b, N = 4; Blumstein and Recapet 2009, N = 8); each track included vocalizations from a single individual and multiple tracks were therefore used in each experiment to avoid the problem of psuedoreplication.
All approaches will be repeated 20 times in every experiment to avoid sample bias.
In all cell experiments, the order of the used substrates (60, 100, 200 nm, controls) was changed randomly in every experiment to avoid artifacts produced by using slightly older cells.
Only salt, from Hiroshima prefecture, was used as flavoring, and no soy sauce, wasabi, ginger, or other ingredients were used in this experiment to avoid any confounding of the taste.
The fungus was grown for at least two cycles in the same medium before the actual experiment to avoid a carryover of signals from other media.
