The exclusion criterion was a diagnosis of hereditary spastic paraplegia with thin corpus callosum in line with an established protocol.
Robert Stubbs was the only witness in the first Zong trial and the jury found in favour of the owners, under an established protocol in maritime insurance that considered slaves as cargo.
Three synthetic peptides representing SPANX-N (EQPTSSTNGEKRKSPCESNN; positions 2 21), SPANX-B (ANEANEANKTMP; positions 21 32), and SPANX-C (SNEVNETMP; positions 18 26) were conjugated to Keyhole Limpet Hemacyanin and used as immunogens in rabbits according to an established protocol [15].
CNS demyelination was induced by cuprizone in mice according to an established protocol.
Acute (day 24) and chronic (day 35 and 55) airway disease was induced in using an established protocol of extended airway challenge 3. Mice also received 25 μg HDM extract (Dermatophagoides pteronyssinus in PBS) (Greer laboratories, Lenoir, North Carolina, USA) or 25 μL PBS intranasally 5 days a week for 5 weeks.
In vivo studies used an established protocol, experimental CtIA, induced in Lewis rats by injection of synoviocyte-packaged C. trachomatis.
Chromosomes from selected His+ recombinant colonies were prepared in agarose plugs using an established protocol [120].
The Work Group therefore only recommends the use of citrate for anticoagulation during CRRT in patients that do not have shock or severe liver failure, and in centers that have an established protocol for citrate anticoagulation.
We used an established protocol for in situ hybridization in 96-well plates (Tomancak et al., 2007) with minor adaptations (see below): we added an over-night wash step after hybridization, incubate the anti-DIG antibody over night and used fluorescent tyramides for probe detection.
To observe the sperm in the antrum, we followed an established protocol [ 42, 43].
In order to study the effects of SIRT1 depletion on MSCs in more detail, we used an established protocol to isolate these cells from bone marrow (Soleimani & Nadri, 2009).
