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Guidelines provided by the NIH were followed in all experimentation.
The samples were stored at 4°C cold room for use in all experimentation.
In all experimentation, laser fluences were measured for each imaging trial, and the mean (n = 500 pulses) energy was used to normalize the PA amplitude.
PCR, polymerase chain reaction; RT, reverse transcriptase; STS, sequence tagged site SG conceived of the study, participated in all experimentation, performed data analysis and wrote the manuscript.
The 'Australian code for the care and use of animals for scientific purposes' was followed in all experimentation and the project was approved by the Garvan Institute and St Vincent's Hospital Animal Ethics Committee.
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Analytical grade reagents and chemicals were used in all the experimentations, procured from Merck (Origin-Germany), unless mentioned otherwise.
In all experimentations, the parameters are set as follows: the step size is chosen as 10−6, filter length is equal to 8, and the parameter B is set to 0.5ts.
The resulting saliva solution was used in all experimentations.
According to the observed nutrient dynamics (Table 3), nitrites increased during the first week before decreasing in the last weeks of the experimentation in all the treatments generally.
In either case, like all experimentation, the process can be repeated over and over again.
All experimentation was done in triplicate.
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