Sentence examples for in alkaline buffer from inspiring English sources

To accelerate the PDLLA degradation, experiments were performed in alkaline buffer solution of pH 10.6.

250 μL of ALP substrate solution (5 mM pNPP diluted in alkaline buffer solution) was added and the plates incubated for 30 min at 37°C.

And it could be easily dispersed in alkaline buffer and completely drawn from the solution to the sidewall of the vial by a magnet field within around 10 s (Fig. 3C).

We prepared nanoparticles differing in morphology from double-hydrophilic block copolymer poly ethylene oxide -block-poly methacrylic acid), PEoxide -block-poly methacrylicescein–[3-coxide -block-poly methacrylicde)] conjugacid, GB176 and GB179, in alkaline buffer.

Electrophoresis was performed in alkaline buffer at 300 mA for 30 minutes at room temperature.

Protoxins were solubilized in alkaline buffer 50 mM Na2CO3, 0.2% β-mercaptoethanol, pH 10.5.

Recombinant 5'Nuc could be readily solubilized in alkaline buffers from pH 8 onwards without the addition of L-arginine.

The embedded cells were lysed in fresh cold lysis buffer [ 48] overnight and then electrophoresed in cold alkaline buffer (pH 13.3), thus the comet assay detects single and double strand DNA breaks, abasic sites, and repair intermediates.

Optimal refolding was obtained in an alkaline buffer containing reduced and oxidized glutathione, and subsequent incubation at 4 °C for 24 h.

The assay of fructosamine with nitroblue tetrazolium (NBT) is based on a color change correlated with the reduction of NBT to monoformazan by Amadori rearrangement products in an alkaline buffer.

To screen for the PLG receptors, exosporium extracts were prepared in an alkaline buffer as described above and subjected to a ligand blot analysis overlaid with PLG and probed with anti-PLG antibody.