Urine samples were collected as described previously [43], in agreement with the protocol established by HUPO (www.hupo.org/research/urine) and EuroKUP (www.eurokup.org).
The procedures used in the study were in agreement with the protocol approved by the Institutional Review Board of Swedish Medical Center (Swedish IRB # 008567).
In circumstances in which it was not possible to obtain informed consent, the requirement for informed consent was waived, in agreement with the protocol.
In agreement with the protocol used, from preliminary mapping a mean inner distance between mate pairs of 307 was found with a standard deviation of 110.
We also generated A431-CR and FaDu-CR cell lines with acquired resistance to cetuximab, through in vivo continuous exposition to the drug followed by tumour explant and in vitro stabilisation of the derived resistant cancer cell line, in agreement with the protocol we previously described (Bianco et al, 2008).
Therefore, the utilization of forest biomass for energy is carbon-neutral (UN-Energy 2007), and in agreement with the Kyoto Protocol (UNFCCC 1997).
In agreement with the standard protocol, the fibroblasts outgrowths and epithelial acini-like structures within the fibroblasts were readily detected in the murine mammary tissue for all treatments tested.
Retrotranscription was performed in a Thermal Cycler (Gene Amp PCR System 2400, Perkin Elmer, Boston, MA, USA) in agreement with the reaction protocol proposed by the manufacturer's: 5 min at 25°C (annealing), 45 min at 42°C (cDNA synthesis), and 5 min at 85°C (enzyme denaturation).
Then, they were washed and incubated with an LSAB 2 kit (DakoCytomation, DAKO Corp., Carpinteria, CA, USA), developed with DAB chromogene, counterstained with Mayer's haematoxylin in agreement with the manufacturer's protocols.
