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In addition to these alignment based features the taxonomic distance of TFs is provided, which is taken from NCBI.
In addition to these alignment based comparisons, we independently assessed the gene composition and putatively identified functional differences between the three strains using RAST [ 51].
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In addition to these two alignments, we prepared new alignments for those taxonomic groups with at least 10 sequences for both cpn10 and cpn60 proteins (sample sizes in Table 1): Actinobacteria, Bacteroidetes/Chlorobi group, Cyanobacteria, Firmicutes, all Proteobacteria together, and Spirochaetes.
In addition to these motifs, the amino-acid alignment generated with MAFFT [ 25] enabled us to identify several conserved amino acids, including a cysteine residue present in the N-terminal part of all molecules, a threonine residue in TM1 and two other cysteine residues located in the first external loop (Additional file 10).
In addition to these methods, ITS1 and ITS2 alignments were constructed using the MAFFT version 7 (http://mafft.cbrc.jp/alignment/server/) [ 113] and Gblocks version 0.91b (http://www.phylogeny.fr/version2_cgi/one_task.cgi task_type=gblocks) software programs [ 114– 116] to eliminate poorly aligned and highly divergent regions.
In addition to the alignment column substitution rate, the ancestral amino acid at each site was also predicted.
There are other tools that allow the user to do other things in addition to the alignment of genomes.
In addition to the alignment extractors and format converters, there is a type of tool that acts as a hybrid of these two concepts.
In addition to the alignment, tools exist to improve the quality of an existing alignment, for example, with local indel realignment.
Thus, in addition to the alignment process Bismark determines the methylation state of each cytosine position in the read (Fig. 1B).
Thus the completely annotated sequence in addition to the alignments is available as additional data (Additional file 3).
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