If a two-level full factorial design 2^7 is applied, 128 experiments need to be carried out, in addition to the replicates needed for statistical evaluation of the coefficients for the fitted model and the degree of coincidence of the hyperplane obtained with the true response surface.
In addition to the biological replicates, 2 or 3 technical replicates of each biological sample were performed.
In addition to the technical replicates commonly used in other assays, in cases where the execution of the assay is split into several runs, it is very common for an inter-run calibrator to be used to quantify the variation introduced by the splitting of the experiment and to normalise for it.
In addition to the evaluation of technical replicates for Dataset 1, we used the same evaluation metrics as described in the section "Batch effect assessment" to determine the adequacy of normalization and EB batch correction after normalization and/or EB correction was conducted on each dataset.
The homes, designed by Roberta Washington Architects of Harlem, will have masonry concrete facades in addition to the cornices and stoops "to replicate the look of original brownstones," Mr. Rooney said.
In addition to the ChIP on chip, three replicates of 300 embryos were used in the ChIP assay, which was performed as described previously (Wardle et al, 2006) using the Prdm1 antibody or rabbit preserum.
In addition to the existing scatterplots comparing two replicates each of pre-MBT and MBT Pol IITSS enrichments in Figure 1 figure supplement 1, we have calculated Pearson correlations for the four pre-MBT Pol II replicates and the three MBT Pol II replicates.
In addition to the situation with complete data, we replicated simulations with 25%and50%0% of censored observations.
In addition to the 16 different expression conditions, 8 replicates of the central point were done to define the experimental error associated to this kind of expression process, as well as the curvature, resulting in the 24 different runs.
Data are averaged from two biological replicates performed in addition to the experiment shown in Figure 5E.
