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Antibodies against PCNA work only on paraffin-embedded tissues, [ 17] and detect cells undergoing DNA repair,[ 24] in addition to proliferating cells.
Furthermore, our in situ hybridization data demonstrate that in addition to proliferating interstitial cells, myc2 is also transcriptionally activated in slower cycling gamete precursors and proliferating epithelial cells in the gastric region (cf. Figs 1– 3).
Although early tangential migration from the MGE begins around E11.5 in the mouse, slices prepared from E15 embryos contain, in addition to proliferating precursors, a large number of migrating cells in the developing cortex.
The high percentage of Mcm2-expressing cells compared to Ki67 can be explained by the presence of replication licensed but non-cycling cells in addition to proliferating cells (Stoeber et al, 2001; Blow and Hodgson, 2002).
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We have shown that these biomarkers detect, in addition to actively proliferating cells, cells with growth potential (Stoeber et al, 2001).
Moreover, this allowed assessment of whether drug could accumulate in quiescent cells in addition to the proliferating outer layers.
However, in addition to ATP rapidly proliferating cells need increased amount of precursor metabolites to fuel their biosynthetic pathways [ 5, 27].
We counted the total number of proliferating cells (BrdU+) in the olfactory bulb, in addition to the number of proliferating migrating neuroblasts in the anterior RMS (BrdU/DCX+), the number of DCX+neuroblasts in the granular layer, and the number of Calretinin+periglomerular interneurons.
In addition to the suppression of proliferating cells, the SVZ looses all its doublecortin (DCX -expressing neuroblasts permanently as of DCX -expressing 15 moneuroblastsadiation (Figure 1H).
Chemotherapeutic agents, in addition to tumour cells, also target proliferating stromal cell infiltrates, which constitute the majority of the cell populations within HL lesions (Kuppers & Rajewsky, 1998).
In addition to pregnancy, mammary glands also proliferate extensively at the onset of puberty and, in these glands, the site of intense mitotic activity resides in specialized structures called the terminal end buds [ 12].
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