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In addition to insertion and deletion modifications, we demonstrated the elongation of inserts via the fusion of two DNA fragments even though they were initially oriented in opposite directions in the BAC vector, thus providing a method for the construction of giant recombinant DNA fragments.
Given the sequence similarity of many promoter elements, homologous recombination mechanisms could be expected to strongly contribute to promoter shuffling, in addition to insertion or deletion events promoted by mobile genetic elements.
In addition to insertion mutants, we generated three site-specific mutants based on prior studies of dnaQ.
Our work reveals that in addition to insertion, deletions and SNPs, the CTn 5 element has moved from its original location within CD1844 to the rumA gene in our isolate.
In addition to insertion and deletion probabilities that vary along the profile, the improvement over, e.g. PSI-BLAST comes from a number of other innovations, including use of the forward algorithm instead of Viterbi (Durbin et al., 1998) and a better algorithm for estimating a profile from a given alignment.
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These results indicate that in the tgε26 allele, in addition to the insertion at Sstr5 locus, the hCD3ε transgene is inserted between the Metrn and Fbx116 loci without disrupting neighboring sequences.
In addition to ad insertion, DirecTV will be using the company's monetization rights management technology, according to FreeWheel co-founder Doug Knopper.
In addition to this insertion, we also found a high confidence variation in the gerH mutant at BAS2094 that encodes a heat shock protein of the hsp20 family.
In addition to the insertion and 5 synonymous mutations, it contains 2 non-synonymous but conservative mutations.
In a few cases, the functional order of rRNA fragments has been rearranged in addition to the insertion of non-rRNA gene fragments.
In addition to the insertion, a large fragment of Xq26.3, encompassing the 3' region of DDX26B (exons 9-16) and the CT45A1 gene, was deleted.
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