For some DMD exons, skipping of additional adjacent exons was observed, either exclusively or in addition to skipping of the targeted exon only.
In addition, another skipping method is presented based on the average motion cost among all previously examined search positions.
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Furthermore, in contrast to PNA2406, PNA2512 induced skipping of exon4 in addition to intron skipping, Figure 7C).
In addition, by RT-PCR, analyses of several human caspase genes indicate that these In100-containing caspase genes indeed undergo alternative splicing, with the skipping of exons immediate upstream of their In100-like elements.
In addition, exon-skipping mutations may be caused by relatively small intragenic insertions, deletions or duplications.
In addition, skipping of exon8 has also been reported to lead to an alternative transcript [ 35].
In addition to exon skipping, we chose three further sample genes with other types of alternative splicing, which also lead to changes in protein properties.
Conversely, the antisense approach failed to correct the F9 exon 2 5′ss mutations, and in addition induced complete skipping of exon 2 (Fig. 3C, lanes 2 11).
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