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This is the basis of the acid growth theory (Figure 3). Figure 3 Auxin or auxin-like substances present in HS in active form (~A) activates H + -pumping ATPase at plasma membrane.
We previously reported a novel esterase, Est25, which was expressed in active form in Escherichia coli, and showed high hydrolyzing activities for various substrates.
Thus, Lpa is expressed in active form at both 37°C and 20°C, and its activity at a range of temperatures suggests that it could be used as a probe of in vivo lipoyl protein inactivation in organisms other than E. coli.
In order to determine the proportion of activated TGF- β1 in the SCC supernatants, we repeated the ELISA comparing acid-activated conditioned medium (in which all TGF- β1 is in active form) with untreated samples.
Several cell types have been shown to release miRNAs that can be transferred in active form to recipient cells, where they can exert their regulatory activity on target genes [ 9, 17, 18].
Our hypothesis is based on a possible double action of hypothyroidism and thyroxine intake: the subclinical TSH increased levels and its possible circadian oscillation could stimulate the endometrial TSHRs (increasing type 2 DIO activity); the circulating levels of exogenous thyroxine could be locally metabolized in active form by type 2 DIO stimulating ERs.
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If these are included in the calculation, a total of 34% of the drug was excreted in active forms.
Complement C1r subcomponent-like protein, mannan binding lectin serine protease 2 and myeloblastin (proteinase 3) were also identified in active forms.
Indeed, treating fish keratocytes with MnCl2 resulted in a profound increase in active-form integrinβ1; however, the increased active-form integrinβ1 induced by such a chemical was found to be located over the entire lamella (Fig. 2E), rather than near the leading edge, which was the situation when calcium uncaging was applied.
cThe bacterial signal peptide from the protein OmpA was utilised in order to produce the protein in an active form in E. coli as described in [37].
In summary, lines 4, 5, and 10 expressed ScAGU115 in an active form, in the apoplast, and in relatively high amounts.
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