In the passive avoidance test, there was no significant difference in the latency time among groups in the acquisition trial (Fig. 1f).
In the acquisition trial, a rat was placed in the illuminated compartment.
On two-way repeated measures ANOVA there was a significant decrease in escape latency across trials of training in the acquisition trial (trials: F = 5.76, P < 0.0001).
On two-way repeated measures ANOVA there was a significant decrease in path length across trials of training in the acquisition trial (trials: F = 4.28, P < 0.0001).
During the reversal task, on two-way repeated measures ANOVA there was a significant decrease in escape latency across trials of training in the acquisition trial (trials: F = 4.39, P < 0.0001).
During the acquisition task, on two-way repeated measures ANOVA there was a decrease in escape latency across trials of training in the acquisition trial (trials: F = 10.20, P < 0.0001), with a difference between groups (F = 5.92, P < 0.0001), which was not influenced by interaction of training trials and groups (F = 0.67, P > 0.05).
On two-way repeated measures ANOVA there was a decrease in escape latency across trials of training in the acquisition trial (trials: F = 11.47, P < 0.0001), but there was no difference between groups (F = 0.64, P > 0.05) and no interaction of training trials and groups (F = 0.86, P > 0.05).
On two-way repeated measures ANOVA there was a significant decrease in path length across trials of training in the acquisition trial (trials: F = 2.79, P < 0.01), but not between groups (F = 0.99, P > 0.05), and no effect of interaction of both factors was detected (F = 1.13, P > 0.05).
In the acquisition trial, all rats were allowed to explore both the compartments for 5 min.
In the acquisition trial (14 days after the operation), we found no difference between the intact and other groups in the step-through latency (STL, data not shown).
