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All you have to do is pour it in according to the instructions, and the solution will do the rest.
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Plasmid transfections were performed using Fugene6 (Roche Applied Science, Indianapolis, IN) according to the manufactur′s instructions.
Double antibody sandwich ELISA was used to determine the concentrations of IL-1 β, IL-6, IL-10, and TNF- α in the plasma and in BALF, according to the instructions in the assay kit (Shanghai Senxiong Technology Company, Shanghai, China).
The assays were performed in the Public Health Laboratory in Amsterdam according to the instructions of the manufacturer.
Something like the Perroche goat's cheese from Neal's Yard Dairy is ideal; or warm some goat's or sheep's milk, stir in rennet according to the instructions, and strain overnight in muslin: easy and homemade.
Sow in drills, according to the instructions on the packet.
The classicist believes that cognition resembles digital processing, where strings are produced in sequence according to the instructions of a (symbolic) program.
Proteins were extracted using TriPure Isolation Reagent (Roche Diagnostics Corporation, Indianapolis, IN, USA) according to the instructions of the manufacturer.
Specific enzyme immunoassays (Biotrin International, France) were used to detect anti-Parvovirus IgG and IgM in sera according to the instructions of the manufacturer.
[DTPA]-octreotide was labelled with In according to the manufacturer's instructions (Mallinckrodt Medical B.V., The Netherlands).
The proliferation assay was performed with MTT Cell Proliferation Kit (Roche Diagnostics, IN) according to the manufacturer's instructions.
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