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In this study, the ACB-AbR, which has been selected more than 135 generations, was used to detect the Cry1Ab resistance-relative genes in ACB.
However, some questions still remain to be further investigated, such as in which tissues or cells these genes are silenced and whether the proposed receptor mediated endocytosis or the transmembrane channel-mediated uptake are the mechanisms leading to the production of RNAi effect as well as the pathway of RNAi effect leading to larval developmental stunt and death in ACB larvae.
We identified genes that are potential candidates for conferring Bt resistance in ACB.
This study identified differentially expressed unigenes related to general Bt resistance in ACB.
Presence of the two allelic classes in ACB could represent ancestral polymorphism.
To our knowledge, this is the first comparative transcriptome study to discover candidate genes involved in ACB Bt resistance.
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Cry1Ab exposure resulted in large alterations of the ACB transcriptome profile, including 636 unigenes being up-regulated and 3,157 being down-regulated in ACB-AbR.
Finally, GPI-anchor biosynthesis was found to be involved in the significantly enriched pathway, and all genes mapped to the pathway were substantially down-regulated in ACB-AbR.
Of these, 47 DEUs were down-regulated in ACB-AbR.
The increased proteolytic activity in ACB-AbR could reflect a higher ability to degrade toxins.
Compared to ACB-BtS, annotated proteases, chymotrypsin/trypsin, were over-transcribed in ACB-AbR.
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