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Amplification of repetitive sequences is therefore frequently used in absolute quantification but problems occur in relative quantification as the number of repetitive sequences is unknown.
It is desirable that in the near future, the final standard uncertainty in absolute quantification in complex phantoms be well below well 5% to ensure the uncertainty in clinical absorbed dose estimations comply with the requirement of 5% to a reference point.
But even among these techniques, the standards used differ, resulting in important discrepancies in absolute quantification [ 14].
This error in absolute quantification of HPV gene copy number by qPCR should be anticipated in future studies.
Even though these result in absolute quantification of FDG influx, they are limited in calculation of 'real' MRglc due to the indefinite value of the LCFDG.
However, we found that gross changes in absolute quantification were seen for relatively small changes in Ft following theoretical evaluations (data not shown).
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In order to directly validate our stoichiometry estimates, we devised a spike-in absolute quantification (AQUA) approach using heavy-isotope-labeled peptide standards (AQUA peptides) (Gerber et al, 2003).
Zeintl et al. [5] showed that the uncertainties in the calibration factor are a dominant factor in the variability of absolute quantification in SPECT imaging.
Two uniplex molecular beacon-based real-time assays were carried out in triplicate for absolute quantification of CCR5 copies and HIV-1 STS DNA copies in clinical samples, as described above for the standards.
No rigorous examination of accuracy has thus far been provided in terms of absolute quantification.
In contrast to absolute quantification, relative quantification is not influenced by artefacts during sample preparation and it provides the means to detect small expression differences.
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