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The samples were registered in a zero background sample holder to avoid external background interferences.
At present the sensitivity and broad applicability of radiolabelling to biological samples followed by 2D separation still offers a competitive alternative in a zero background.
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We raised the kita-GFP-RAS line in a mitfa-/ background (a mutant called nacre [31]) and observed that these embryos, although initially expressing the GFP HRASV12 transgene in melanocyte progenitors (figure s3a) by 3 dpf were devoid of melanocytes (figure s3b, e) and did not develop any tumor by 9 months of age.
We also grew kita-GFP-RASV12 transgenic fish in a tp53-/ background [25].
(5) Strong upconverting signals can be detected against a virtually zero background in the context of cells.
The ZeBaTA system introduces the TA cloning into plant binary vectors and results in zero background vectors [ 12].
This measurement resulted in a zero mean ambient light background level (standard deviation: 1.3 counts/pixel).
(D )–(F ) Population data reporting the slope of each parameter on a log log scale in three background regions.
The sorption of Eu III) was then investigated by the batch method, in the three background electrolytes, as a function of pH.
As before it is useful to summarise the data on the different variables determining A level performance by means of a path analysis, and Figure 6 shows a LISREL model for the determination of A level points in terms of nine background variables.
In 2009, 14 million background checks were performed, a jump from 12 million in 2008.
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