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48 hr after viral infection, medium was aspirated and 1 mM CPA was added in a vol of 60 μl/well.
Cells (10) were injected using a Hamilton syringe (Sigma-Aldrich, Milan, Italy) at a depth of 3.5 mm in a vol of 2 μl.
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Cell pellets corresponding to 5 mL of this culture were resuspended in 400 μL isobutyric acid/1 M ammonium hydroxide in a 5 3 vol:vol ratio.
These premixed solutions were prepared by blending 50 wt % PB- hPEO and 50 wt % PB- dPEO in benzene, freeze-drying, and dissolving the blended polymers in a 64 vol % D2O/36 vol % H2O mixture.
The specimen was cleaned ultrasonically in ethanol for 10 min and chemically polished in a 10 vol.% HF and 60 vol.% H2O2 solution for 3 min. All electrolytes were prepared from reagent-grade chemicals and deionized water.
The New Yorker, March 3, 1934 P. 17 Talk story about Urban Shirk, who read the eleventh edition of the Encyclopaedia Britannica, starting at "A" in Vol. 1 and winding up with "Zygote" Vol. 23, in four years and a half.
Talk story about Urban Shirk, who read the eleventh edition of the Encyclopaedia Britannica, starting at "A" in Vol. 1 and winding up with "Zygote" Vol. 23, in four years and a half.
Prior to coating the precursor, three different Ti-pretreatments including grinding with emery paper, etching in a 50 vol.% HCl solution and etching in a 19 g/L NH4HF2 + 5 vol.% HNO3 solution were performed.
Thus it kicked off with, among others, the magical Ivor Cutler and his Life in a Scotch Sitting Room, Vol II.
The titrations were performed in 2-propanol or in a 50 50 vol.% mixture of 2-propanol and toluene.
40S·eIF1·eIF1A·mRNA complexes were preassembled for 2 hr at 26°C in a reaction vol of 60 µl.
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