In particular, high-throughput screening of TF binding specificities combined with accurate TF binding prediction provides a viable, condition independent, alternative to somewhat complex ChIP-chip experiments [20].
The cleaned seeds are either retained in fungus gardens or later discarded in viable condition in rubbish heaps [25], [26].
In a subset of patients with an available frozen cell sample stored in viable conditions, surface phenotypes were evaluated on thawed PBMCs by flow cytometry (FACS Sort Becton-Dickinson, San Josè, California, USA) using directly labeled antibodies (fluorescein isothiocyanate [FITC], phycoerytrin [PE], and Peridinin-chlorophyll-protein complex cyanin 5.5 [PerCP Cy5.5]).
While liquid media did not perform well in this study, they should not be discounted as a viable culture condition in other circumstances.
Following this episode continued overflow from the upstream chamber restores viable conditions in the downstream chamber by diluting out the lethal agent and replenishing it with living cells from the upstream chamber.
Seeds can remain viable in a dormant condition for a few days or, in some species, for hundreds of years.
Also, no N− alleles were recovered, which in this case was surprising as such loss-of-function alleles generated by NHEJ would be expected to be viable in a heterozygous condition (see explanation below).
The candidacies of O'Malley, Sanders, and Webb, and probably others, might be seen as an updated version of the Carter experiment to discover, as Webb recently told George Stephanopoulos, whether it's "possible to conduct a viable campaign" in conditions that, in 2016, seem to make the venture preposterously out of reach.
The candidacies of O'Malley, Sanders, and Webb, and probably others, might be seen as an updated version of the Carter experiment — to discover, as Webb recently told George Stephanopoulos, whether it's "possible to conduct a viable campaign" in conditions that, in 2016, seem to make the venture preposterously out of reach.
