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In a typical preparation, a mixture of the as-prepared powder and deionized water (20 mg ml−1, 50 ml) was heated and kept boiling for 5 min. The boiled broth was allowed to cool down and then decanted.
In a typical preparation, a 15 μM (final concentration) NC probe solution was prepared by adding 12.5 μL of 1.2 mM NC probe (Supporting Information Tables S1 and S4) to 940 μL of 20 mM sodium phosphate buffer (pH 6.7).
In a typical preparation process, several photoelectrodes (PE) with equal thickness but different compositions were prepared.
In a typical preparation procedure, pseudoboehmite and water were vigorouly stirred to form a slurry.
In a typical preparation procedure, a Ti buffer layer with thickness of 50 nm was deposited on the silicon substrate.
In a typical preparation, the TEOS was dissolved in the ethanol using magnetic stirring for 15 min.
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A typical preparation started with 300 mg of HA-TBA in 50 mL of (CH3 2SO CH3OH (3:2 v/v) mixture.
induction time, a typical preparation from 1 liter of culture medium yielded ∼12 mg of purified BmrC/BmrD in detergent solution, with an ATPase activity of 231±17 nmol·min−1·mg−1 (0.56±0.04 s−1; n = 5 measurements with two different purification batches).
For a typical preparation, the titre was approximately 2- to 7 × 10 TU ml−1.
A typical preparation yields 50 mg of purified RDL1 from 10 g of cells.
In a typical film preparation, anodic aluminum oxide (AAO) membrane templates were placed in a glass petri dish, and a drop of AuNP suspension (ca 0.02 mL; standard solution, see SI-1 in Additional file 1) was added onto each membrane, followed by a natural drying process.
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