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A triple overlapping PCR was performed with equimolar amounts of the flanking PCR fragments and a twofold amount of the kanamycin cassette PCR product using PhusionFlash High-Fidelity PCR Master Mix (Thermo Fisher Scientific, Waltham, USA).
Addition of MIF to proliferating DU-145 prostate cancer cells resulted in a twofold increase in the relative amount of active MMP-2 [ 54].
An equivalent amount of 111In chloride resulted in a twofold decrease from 100±5%5% to 46±4%4% only.
Doubling the amount of metallic iron in the bioceramsite media resulted in a twofold increase of eubacteria in the MBAF, but a decrease in the ratio of anaerobic ammonia-oxidizing bacteria to total bacteria.
Thus, if we assume that the slip deficit has accumulated for 200 or 300 years, it would create a twofold or threefold amount of slip in the same rupture area.
So we tried to fix this problem in a twofold manner.
Harmful interference is defined in a twofold way.
Clearly, a twofold differential expression of genes may not necessarily result in a twofold change in protein expression [55].
The KORINNA study is unique in a twofold way.
Test samples were tested in a twofold serial dilution.
In conclusion, our pooled study resulted in a twofold increased risk of lung BAC in ever smokers when compared to never smokers, and in evidence of a dose response relation according to duration and amount of smoking.
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