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Double-coincidence count data are 16-bit depth, for 16 SV steps, azimuthal 16 channels and 8 m/q bins, resulting in a total size of 4096 bytes.
The sequence is contained in the BAC clone BAC 273i4, and encompasses 17 exons and 16 introns in a total size of 26642 bp.
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Hence, 186,324 unigenes was obtained, ranging from 201 bp to 13,149 bp in size, with a total size of 166.7 Mbp.
This resulted in 885,704 contigs with a total size of approximately 38 Mb.
Sequencing and assembly resulted in a genome of a total size of 5,242,029 bp distributed over 159 contigs.
In total, 28 NC regions have been identified in H. momus mtDNA, with a total size of 1,457 bp (9.21% of the total genome).
Among the 572 segregating markers, 569 were mapped in nine linkage groups, with a total size of 669.6 cM (Additional files 2 and 7).
Then we sampled 193 pregnant women in each trimester of pregnancy resulting a total size of 579 pregnant women.
The grapevine genome is diploid and organized in 38 chromosomes (n = 19), with a total size of ~487 Mb.
Therefore, a 20%% inflation factor was included to accommodate missing data, withdrawals, an imbalance in cluster sizes, resulting in a total sample size requirement of 98 patients.
To account for potential attrition, we will inflate the sample size by 10%, resulting in a total sample size of 39 with 13 participants allocated to each group.
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