These data strongly support our model in which a tight interaction between spartin and the WW-I and WW-II domains of AIP4 (Additional file 3) disrupts a weaker intramolecular interaction, which leads to increased catalytic activity of this E3 ligase.
We further assumed —based on evidence in favour of a tight interaction between the Notch and Wnt signalling pathways in mice [19]— that this gene and another subject to regulation by FGF and/or Wnt (W) interact by up-regulating each other's expression.
One of the major mechanisms used to control the activity of peptidases in mammals is a tight interaction with natural peptidase inhibitors such as serpins, cystatins and tissue inhibitor of metalloproteinases (Rawlings et al., 2004).
Once formed, the spontaneous dissociation rate of the MCC APC/C complex is small as observed in vitro and in mitotic extracts, indicating a tight interaction (Reddy et al, 2007).
To master the increasing complexity of these devices a new design flow has to be put in place, which enables a tight interaction among system companies and system integrators.
However, the fact that the linker is flexible in its position relative to the ring we think argues against a tight interaction; in that case, we would have expected to be able to resolve the linker at AAA4 without having to use 3D sorting.
Nevertheless, most related work has been based on simplified assumptions on the underlying vehicle traffic dynamics, which has a tight interaction with VANET in practice.
It is expected that the elimination of the buffer resulted in a tighter interaction between the different components.
Indeed, GlcNAcstatin inhibited a bacterial OGA orthologue in the picomolar range, and structural analysis revealed a tight interaction between the catalytic acid and the (presumably protonated) imidazole [ 41].
