Exact(9)
Total RNA was precipitated with 2-propanol, washed once with 70% ethanol, and re-suspended in a suitable volume of DEPC-treated water.
Pellet and resuspend in a suitable volume of 1M sorbitol plus 20mM HEPES.
Following the treatment cells were pelleted, washed and finally resuspended in a suitable volume to perform chemotaxis.
Unbound antibodies were removed by washing with PBS, and cells were resuspended in a suitable volume of buffer.
The use of cubosomal dispersion was the possible practical solution to attain the required dose of 5-FU in a suitable volume for subcutaneous administration.
To remove 2-mercaptoethanol, membranes were then washed three times for 10 min in a suitable volume of PBS-Tween 20 (0.1%).
Similar(51)
Proper volumes of MST WS2 (10 µg/mL) were diluted with a suitable volume of RLMs matrix to prepare eight concentrations: 5, 10, 15, 20, 50, 100, 150 and 200 ng/mL in a final volume of 1 mL.
Biomass (20 50 mg dry biomass) was collected by centrifugation of a suitable volume of culture broth, washed with an equal volume of de-ionised water, and stored at −40 or -80°C in Eppendorf Safe-Lock micro-centrifuge tubes.
Therefore, FBA of 75 mL was a suitable volume for the reaction.
Samples were suspended in the suitable volume of ethanol prior to HPLC injection.
The organic component was once again removed by evaporation in a vacuum centrifuge and peptides were resuspended in a suitable nanoLC injection volume (typically 3 10 μL) of 2.5%ACN/0.11% TFA.
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