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Whole lungs were excised, cut into small pieces (max 0.5 cm diameter) and transferred in a sufficient volume of RNAlater (Ambion) and frozen in liquid nitrogen.
Once, this has been achieved, the brain is immersed in a sufficient volume of 10%% neutral buffered formalin and fixed for 48 h prior to further trimming and gross examination [ 33].
Because of the optical complexity of skin, which contains numerous reflective elements, two essential options exist for in vivo imaging: either sufficient optical resolution is achieved in a sufficient volume of skin to allow diagnosis, with accuracy comparable to that of histopathology, or in vivo methods have to rely on pattern recognition at a lower magnification.
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44 While in-house data trends and research 135 suggests that there is some risk in players completing high volumes of high-speed running, the risk in not performing a sufficient volume of such training contributing to reinjury upon returning to play may be even higher.
This strain had been isolated in 2001 from a sentinel hamster in coastal Chiapas, Mexico, and passaged once in Vero cells to generate a sufficient volume of high-titer virus for experimentation.
This study has shown that application of explicit measurable criteria can lead to improved designs for sampling EQ-5D health states in a valuation exercise, provided a sufficient volume of real-life patient EQ-5D responses is available as a basis for identifying appropriate health states.
The defect in Group II was filled with a sufficient volume of the hyaline cartilage tissue rich in proteoglycan and type-2 collagen.
In our study, patients were administered a sufficient volume to avoid hemorrhagic shock.
One of the main problems with this technique is that, with a perfusion rate of 0.3 2 μL/min, the time taken to collect a sufficient volume for assay in a bedside analyser can take 30 min to an hour.
Once a sufficient volume of magma forms in the subsurface, it tends to rise from its own buoyancy.
Plasmid DNA was mixed with Lipofectamine 2000® (Invitrogen) in a 1 2 v:v ratio in sufficient volume to mix well with the TCP particles.
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