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PCR-generated DNA fragments were first purified from agarose gel using GFX PCR DNA and Gel Band Purification Kit (Amersham Bioscience) and then employed in a subsequent overlap extension reaction to obtain the resulting fusion products.
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In another subsequent overlap, Wilson became the MP for the Huyton area of Liverpool, where his political backers and admirers included Shankly.
In the subsequent overlap of cores (Fig 5c and 5d) we observe the presence of the important signaling domains zf-CCCH, zf-C2H2 and zf-C3HC4 from the zinc-finger family.
The PCR products were pooled and used as a template in the subsequent overlapping PCR.
This method was performed in two steps [ 26]: 1) complementary oligodeoxyribonucleotide primers were used to generate two DNA fragments (named "a" and "b") having overlapping ends; and 2) these fragments were combined in a subsequent 'fusion' reaction in which the overlapping ended anneal.
In a subsequent step, all cCNV segments are annotated for their overlap with the gene regions in refSeq and the CNV regions in the [ 5], referred to as 'Sanger' in the rest of the text.
Here we have focused on hard partitions and the flow of random walkers, but in a subsequent paper we will demonstrate the natural extension of the map equation to overlapping partitions and generalized flows.
In a subsequent merging step, predicted and existing gene models were merged into a hybrid gene set, retaining predictions that (a) did not overlap with existing gene models or (b) showed a PID improvement of at least 5% over overlapping existing gene models.
(More on this in a subsequent column).
(More on this in a subsequent posting).
More on that in a subsequent post.
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