Briefly, slides were rehydrated in 3X 5 minute washes in xylene followed by single sequential 5 minute washes in 100, 95, and 75% EtOH.
These concentrations were determined simultaneously in a single step using sequential injection analysis with multivariate curve resolution alternating least squares (SIA-MCR-ALS).
Fig. 3 Series of laser irradiation experiments to remove thin pollution crust from marble in single, sequential and synchronous use of the two wavelengths (IR at 1064 nm and UV at 355 nm).
Prior studies have attempted to detect multiple MRI contrast agents in a single scanning session using sequential administration of contrast agents32, ratiometric methods to detect the presence of activatable MRI agents14, and machine learning33.
Therefore, we employed a methodology where cell lysis, mRNA purification and cDNA synthesis occurs in a single well, through the sequential addition of the necessary enzymes and buffers, without intervening cleanup steps, and especially without removing the sample from the well.
In order to corroborate our SimPull observations on the existence of ORC-ORCA-H3K9 KMTs and G9a-ORCA-Suv39H1 G9a-ORCA-Suv39H1 G9a-ORCA-Suv39H1sequentinl IPs.
Heuristic maximum likelihood phylogenetic analyses with 1000 non-parametric bootstrap replications for all nodes were performed in a single run with the sequential version of RAxML Ver. 7.2.8 [ 60, 61] under the GTR+Γ+I substitution model [ 62] as chosen by Akaike and Bayesian information criteria [ 63, 64] implemented in Modelgenerator [ 65].
A short series of 2-alkoxy-4-aminotrifluoropyridine derivatives was prepared from pentafluoropyridine in a single continuous flow process by sequential nucleophilic aromatic substitution.
However, with manual gating, at most two markers can be examined in a single dot plot, and a sequential strategy is often used.
