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For between copy primers the sequences were cut in half and pasted in a reverse orientation to generate properly directed primer sets.
One of the binding sites occurs in a reverse orientation to the other.
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The virus integrated in a reverse orientation relative to the SOX5 gene at Chr1 66,086,819.
The second moiety is the sulfonamide group in AM1, which is in a reverse orientation relative to Pyrabactin.
The PGK promoter that drives expression of the neomycin resistance gene cannot be the origin of ectopic transcripts as it is located about 1.7 kb downstream from the region analysed and in a reverse orientation relative to Xist.
The plasmid pRed-Caps-Lib was created incorporating the AAV8 rep/cap gene expressed from a PGK promoter in reverse orientation to the dsRed gene expressed from a CMV promoter.
Some templates produced a double sequence close to the P0 site, these were sequenced using another primer that binds in the reverse orientation to a conserved region in the middle of the 16S region (095P: 5'-TAC GGC GTG GAC TAC CAG-3').
The ribosomal DNA (rDNA) BAC consisted of 14 45S ribosomal transcription units with a portion of one unit in the reverse orientation to the other 13 (Additional file 12: Figure S7A).
The rearrangement suffered by the T-DNA during the integration process placed a truncated 35S promoter in reverse orientation to the tagged gene.
In all of the studied species belonging to Antilopinae, there was one insertion event of the Bov-A2 element in the exact same location and yet in reverse orientation to the one described in the literature.
The phaC Bs was also cloned in reverse orientation to lac promoter in pBBR1MCS-2 to confirm the expression under its native promoter.
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