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A decrease in cross section is seen for tilted samples due to the reduction in channelling, resulting in a quantification error, if this is not taken into account.
Chen, I.V. Turko, 2014) [2], "Natural flanking sequences for peptides included in a quantification concatamer internal standard" (C.S. Cheung, K.W. Anderson, M. Wang, I.V. Turko, 2015) [3]) and SpikeTides versus the label free Hi3 approach.
This approach involved the analysis of 40-ml field samples and resulted in a quantification limit based on both sample and lysis buffer volumes.
The primer pairs used for amplicon construction for each gene resulted in a quantification cycle (Cq) [ 17] value of zero or >35 for the nontemplate control.
The qPCR results of clinical samples were only considered valid if the β-globulin PCR resulted in a quantification cycle (Cq) value ≤35 and second derivatives of the amplification plots were calculated to determine the fractional qPCR cycle value.
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In addition, a quantification plasmid allowed for quantification of the bacteria copy number.
This resulted in (ii) a quantification based on the desired levels of staining intensity (= fraction at different intensity levels).
In addition to a quantification of PKS I in diverse environments, our study has also helped to classify unknown proteins in UniRef and improved their annotation.
In this study, a quantification method of delamination was examined with maintaining the merits of FBG sensors.
Hence, in this study a quantification strategy for fluorescence-labeled oligonucleotides was developed to solve these disadvantageous effects.
In this paper a quantification method, which is independent from the tissues and fluorophores types, was presented and analyzed.
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