If an investigator has a primitive stem cell line it must be registered in the Stem Cell Line Registry, https://duke.qualtrics.com/SE/?SID=SV_3VIw7b1qLwNkNBa.
In summary, we demonstrate that BAP1 is necessary for maintenance of melanocyte identity in uveal melanoma cells, and that loss of BAP1 leads to a loss of cell identity and acquisition of a primitive, stem-like phenotype.
While chromosomal abnormalities have not been described in stromal fibroblasts, it could be questioned whether the initial oncogenic event leading to the MPN development might occur in a primitive mesodermal stem cell.
Zhou et al. [42] reported that the expression of Bcrp1, the gene encoding the BCRP transporter protein, is not only highly conserved in various primitive stem cells but also serves as a marker for the side population phenotype.
Necdin (Ndn) preferentially expressed in primitive stem cells, is an important protein in hematopoietic stem cell regulation [ 26].
Initially, we assessed whether AL cells derived from AF-MSCs could dedifferentiate to a more primitive stem cell-like stage.
The hepatogenic marker tyrosine aminotransferase was expressed in similar levels in AF-MSCs and TRAF-MSCs (P=0.1087, Student's t-test), which implies that later cell types have returned to a more primitive stem cell-like stage.
In particular, the most primitive stem cells (from a developmental point of view) are present at very low frequencies in adult tissues.
The effects of this oncogenic tyrosine kinase in the context of primitive stem cells are not known.
Since the 20 neurons we analyzed were all from cultured human neurons differentiated in vitro from more primitive stem cells, we wonder whether the neuronal maturation genes (138) we identified could be used generally to mark neuronal maturity.
