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Electrophoresis can also be used in a preparative mode.
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The injection loop volume was 0.020 cm in analytical mode and 7 cm in preparative mode.
The productivity in the preparative mode of SFC was most influenced by the co-solvent fraction and temperature.
The pump rate was 1.5 cm/min for the analytical column and 31.26 cm/min in preparative mode.
Furthermore, the enhanced filter and lighting configuration ensures a reduced background signal, aiding the capture of high-contrast images that are ideal for publication purposes and for working in preparative modes.
A total of 20 µg purified protein was separated in a preparative gel, followed by blotting and preparation of membrane strips (each containing 1.2 1.4 µg protein).
The fluorescent-labeled product 1 was purified in a preparative chromatographic column.
Purification was performed in a preparative M-N VP250/16 Nucleosil 100-5C18 colusingsing two mobile phases.
Secondly, due to their particle structure and high molecular weight, VLPs can be easily purified in a preparative scale.
In a preparative experiment, NCC 1 (192.9 mg) was adsorbed on silica gel 60 (19.3 g).
The reconstituted supernatant was injected in a preparative HPLC column (XBridge C18, 19 × 250 mm, 5 μm, Waters).
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