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In a parallel experiment to that described above, ICI arrested cells were induced with AP, harvested at 24 h intervals for 4 days and subjected to western blotting and in vitro kinase assays for cdk2 activity.
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In a parallel experiment, NaClO4 was added to enzyme, and aliquots were removed and assayed at various times.
In a parallel experiment, encapsulated islets were exposed to lymphocytes in the presence of free PTX in the culture medium.
In a parallel experiment, p53 was first heated to denaturation for 1 h at 37°C followed by addition of NTD125 (Fig. 3b).
In a parallel experiment, the bands that corresponded to the modified proteins were excised from a Coomassie Blue stained SDS-PAGE acrylamide gel.
In a parallel experiment, the immunoprecipitated proteins were subjected to deglycosylation using N-glycosidase F prior to SDS-PAGE analysis.
In a parallel experiment, UMSCC-10B exhibited less sensitivity to salinomycin treatment, with an IC50 beyond 8 μM (Fig. 2A).
In a parallel experiment, the method was also used to analyze YMDD/YVDD allele mixed samples with above proposed workflow (data not shown).
The non-antioxidant control for MitoQ10, DTPP, had no effect on AMPK activity in a parallel experiment, indicating results can be attributed to the antioxidant action of MitoQ10 specifically.
In a parallel experiment mice were observed for 14 days to assess epithelial damage and lung fibrosis.
In a parallel experiment, the evolution of the rheological properties can be measured (Fig. 3a).
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