In addition, a nuclei filter was used to remove over-segmented nuclei.
Samples were chopped together using a razor blade in the presence of a nuclei isolation solution (High Resolution DNA Kit Type P, solution A; Partec, Münster, Germany).
An additional consideration is that genes featuring poised polymerase at the promoter in many or most nuclei, but are only expressed in a few nuclei, will have weak signals at the body of the gene.
Interestingly, an additional single signal was found in a few nuclei and metaphases (9%), due to the presence of an euploid cell line, which was characterized by a ring(X) chromosome in addition to the conserved one (Fig. 2A and B).
Then, samples were imbedded in mounting medium containing DAPI, a nuclei staining agent (Vector Labs, Burlingame, CA).
To examine the surface barrier function, living worms were incubated for 15 min in buffer containing a nuclei-staining dye Hoechst 33342, which does not usually permeate the worm cuticle barrier.
In Drosophila and other Dipterans, homologous chromosomes are in close contact in virtually all nuclei, a phenomenon known as somatic homolog pairing.
KIAA0101 is predominantly expressed in mitochondria and partially in nuclei, playing an essential role in the regulation of DNA repair, cell cycle progression, and cell proliferation [ 12].
CD41 expression was found in cells with a round nuclei and a thin rim of cytoplasm as well as in cells with abundant cytoplasm.
At high HPPH-Gal-PDT doses, the cellular remnants were fixed to the support as seen for HPPH-treated cells, but differed in that all nuclei show a marked vacuolar structure (Supplementary Figure 1D and F).
