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The molar concentration of test compound producing 50% inhibition (IC50) were calculated for each test compound in a minimum of triplicate and geometric means and 95% CI were subsequently calculated.
In vitro experiments were performed in a minimum of triplicate.
All experiments were performed at least twice, and in a minimum of triplicate wells.
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All experiments were performed in a minimum of triplicates.
All cell culture experiments were analyzed in a minimum of triplicates, and reproducible data representing one of ≥3 independent experiments was demonstrated.
For all above experiments, we ran a minimum of triplicate wells for each condition and repeated experiments in different patient-derived cells whenever feasible.
Average and standard deviations reported represent a minimum of triplicate measurements.
All test results were from a minimum of 9 replicates resulting from a minimum of triplicate replications from 3 separate trials.
All quantitative real-time polymerase chain reaction (qRT-PCR) experiments in cell lines were performed in triplicate, a minimum of three times and included no-template controls.
All experiments were done in triplicate; a minimum of 10 embryos were used for each genotype per experiment.
All in vitro experiments were repeated a minimum of three times, in triplicate.
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