Sentence examples for in a master circle from inspiring English sources

These sequences could be contained in a master circle two or more than two times.

In this study, the mitochondrial genome of hau CMS line, its maintainer line and the normal type line "J163-4" were fully sequenced and assembled into a master circle.

We further used the PCR results to order scaffolds into a master circle.

Results of the PCR amplification are presented in Additional file 2. A master circle was developed for each mitochondrial genome using a 'parsimonious' method [ 24].

A master circle was reconstructed for A, B, and macrocarpa, whereas CMS-E and CMS-G genomes were reconstructed in two contigs (one circular and one linear) (fig. 1).

When it was difficult to judge whether a given contig really appears in the master circle, we checked the existence of the contig in all possible regions by genomic PCR and sequencing (Additional file 6).

In the master circle the break point sections share a 101 bp repeat sequence with 1 mismatch between the two sequences which may be the footprint of a homologous recombination (see Additional file 2).

Assembly of the approximately 690 kb milkweed mitochondrial genome from the same individual that we used for plastome sequencing revealed that it contains sequences homologous to the inserted segment in the plastome (fig. 1 A), which are split between two regions of 1,091 and 1,401 bp, and separated by 55,845 bp in the master circle of the contemporary A. syriaca mitochondrial genome.

Several large segments of the mitochondrial genome were repeated in reverse orientation in the master circle resulting in duplication of approximately 350 kb in the master circle.

We noted that all mitogenomes are in the master circle conformation and all our analyses were based on this conformation.

In this master circle (MC) molecule, there were four large repeat sequences of more than 20 kb.