It consists of a compressor, a condenser, a throttle or expansion valve, an evaporator, and a working fluid (refrigerant), such as carbon dioxide, ammonia, or a halocarbon.
A transparent electrorheological (ER) fluid was fabricated by dispersing urea-modified silica nanoparticles in a mixture of halocarbon oil and silicone oil, whose refractive index could be tuned to be the same as that of the dispersed particles.
Ovaries from well-fed females were dissected and individual egg chambers separated from ovarioles using a dissecting probe in series95 halocarbon oil (KMZ chemicals) on 22 × 40 cover slips, #1.5 (Menzel-Gläser).
To test whether mechanical pressure is able to prompt and propagate a wave of Ca2+, we applied pressure with a glass rod to the outside of an oocyte mounted in halocarbon oil (Fig. 4A,B).
Embryos were then desiccated at room temperature, covered in 700 halocarbon oil and injected with a 1 mg/ml solution of dsRNA or siRNA using Eppendorf's sterile Femtotips® needles.
GFP-expressing embryos were staged to cycle 10 in halocarbon oil using a dissection microscope.
Three- to 5-day-old adults of the appropriate genotype from each cross (in some experiments following thorax measurement) were placed on a slide containing a thin layer of silicone vacuum grease (Beckman, Fullerton, CA) and mounted in Halocarbon 700 oil under a coverslip supported by capillary tubes.
Live embryos were dechorionated and mounted in the halocarbon oil 10S (commercialized by VWR, Pennsylvania, USA).
In order to visualize the complementation between split Venus fragments, living embryos were dechorionated and mounted in the halocarbon oil 10S (commercialized by VWR, Pennsylvania, USA).
The oocytes were teased in Halocarbon oil 700 (Fisher) on a glass cover slip.
