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The ductal approach to breast cancer, encompassing nipple aspiration, ductal lavage and duct endoscopy, allows assessment of breast ductal epithelial cells and their local microenvironment in a graded process of increasing invasiveness.
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Sections (2 μm thick) were deparaffinized in xylene, dehydrated in a graded series of ethanol, and processed for antigen retrieval in 0.01 M citrate buffer using a Pascal pressure chamber (DAKO, Glostrup, Denmark).
The pieces were dehydrated in a graded ethanol series and routinely processed for embedding in paraffin, sectioned at 5 μm, and subsequently stained with hematoxylin and eosin (H&E).
When decalcification was complete, the samples were dehydrated in a graded series of alcohols from 70% to absolute and then processed for paraffin embedding.
Neutrophil CD64 expression is regulated in a graded fashion in parallel with the degree of inflammatory response to a significant clinical process of infection or tissue injury [ 10].
Muscles were dehydrated in a graded series of ethanol and embedded in epon resin.
After rinsing in 0.1 M Na cacodylate buffer, they were sequentially dehydrated in a graded acetone series.
The sections were first deparaffinized with xylene and then rehydrated in a graded alcohol series and allowed to air dry.
After the differentiation switch is triggered, insulin controls fat accumulation in a graded fashion.
Then, every proper graded submodule of M is contained in a graded prime submodule.
We found an increased frequency of injury reduced the trigeminal thresholds in a graded manner.
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