Sentence examples for in a fragmentation from inspiring English sources

PolyA RNA was isolated from total RNA using the Dynabeads mRNA purification kit (Life Technologies, Grand Island, NY) and fragmented in a fragmentation buffer (Life Technologies) at 70° for 90 sec to 200−300 nt fragment sizes.

Labeled cRNA was fragmented in a fragmentation buffer during 35 min at 94°C.

The mRNA was fragmented into short fragments ~200 nt pieces by incubation in a fragmentation buffer under elevated temperature (The Beijing Genomics Institute).

For amplification-based sequencing, messenger RNA (2 µg) was fragmented at 85°C for 5 min in a fragmentation buffer (Ambion) and converted to single stranded cDNA using SuperScript II reverse transcriptase (Invitrogen), followed by second-strand cDNA synthesis using Escherichia coli DNA polymerase I (Invitrogen).

Briefly, 825 ng each of Cy3 and Cy5 labelled aRNA was fragmented to nucleotide lengths of 60 – 200 in a fragmentation mix and then added to the hybridization buffer.

Starting from 10 μgs of total RNA, 16S and 23S rRNAs were removed using the MICROB Express™ kit (Ambion), after rRNA depletion, RNA was fragmented incubating the sample at 82°C for 6 mins in a fragmentation buffer (40 mM Tris Acetate pH 8.1, 100mM KOAc, 30 mM MgOAc-4H20).

Low dose of sodium arsenite does not induce DNA fragmentation in A) BEAS-2B cells and B) HeLa cells.

Their numbers may be small in Australia so far and that may, in part, indicate a fragmentation of the local far right.

a In silico fragmentation can be used to identify and rank unknown MS/MS spectra by matching theoretical fragments to experimental MS/MS spectra.

Low-energy collision-induced dissociation (CID) of the precursor ion, that is, sodiated molecule, resulted in a clear fragmentation to one specific fragment ion [Ti L 2+Na]+.

Other analysts see things differently and project a fragmentation in the ultra-portable computer world.